Rapid detection of Salmonella with Recombinase Aided Amplification（沙门）-浙江检验检疫

Salmonella, a foodborne disease causing pathogen, is often trans-mitted through contaminated food and hence, represents a major publichealth threat worldwide (Saikia et al., 2015). Rapid and reliable de-tection of Salonela remains challenging. To date, identification ofSalmonella has relied mainly on traditional methodologies such as im-mune-testing or various molecular detection approaches. Traditionaltesting is particularly time-consuming and expensive (Bhutta, 2006WHO,2003).Similarly,immunological approaches such as enzyme.linked immunosorbent assay(ELiSA), immune colloidal gold staining(1CG),immunomagnetic bead separation (IMS)and immuno-fluorescence (lF), are also time-consuming (Bolton et al., 2000; Coburnet al., 2007; Fan et al., 2015; Kumar et al., 2008; Nicholas and Cullen,1991). Numerous PcR-based molecular detection systems have beenrecently developed, offering significant advantages (Daum et al., 2002;Dobhal et al., 2014; Kapley et al., 2001; Kurowski et al., 2002; li andMustapha, 2002;Malorny et al,2003). However, these systems re.quired testing of multiple primer sets, and thus are still time-consuming(Nagamine et al., 2002; $risawat and Panbangred, 2015; Yang et al..2015).

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