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Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7（腺病毒）-CDC
Background: Human adenoviruses are a common group of viruses that cause acute infectious diseases. Humanadenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia. A reliable and practical method forHAdV typing in clinical laboratories is lacking. A simple, rapid and accurate molecular typing method for HAdV mayfacilitate clinical diagnosis and epidemiological control.Meth ods: We developed and evaluated duplex real-time recombinase-aided amplification (RAA) assays incorporatingcompetitive internal controls for detection of HAdV 3 and HAdV 7, respectively. The assays were performed in a one-step in a single tube reaction at 39° for 20 min.
Results: The analytical sensitivities of the duplex RAA assays for HAdV 3 and HAdV 7 were 5.0 and 14.8 copies perreaction, respectively (at 9596 probability by probit regression analysis). No cross-eaction was observed with othertypes of HAdV or other common respiratory viruses. The duplex RAA assays were used to detect 152 previously-defined HAdV-positive samples. These results agreed with those obtained using a published triplex quantitative rea-time PCR protocol.
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